AKTIVITAS ANTIOKSIDAN DARI DAUN BINTANGUR (Calophyllum inophyllum L.) TERHADAP RADIKAL BEBAS DPPH (1,1 Difenil-2-pikrihidrazil
DOI:
https://doi.org/10.33759/jrki.v4i1.200Keywords:
Antioksidan, DPPH, Bintangur, Calophyllum inophyllum L.Abstract
Antioxidants are compounds that can inhibit oxidation reactions by binding to free radicals and highly reactive molecules. Bintangur contains saponins, tannins, flavonoids and alkaloids. In the presence of flavonoids, the purpose of this study is to understand the antioxidant activity of ethanol extracts against DPPH free radicals, and the IC50 parameter has the highest antioxidant activity. Use ethanol to macerate Bintangur leaf powder. Analyze the content of secondary metabolites in ethanol extracts. Use a spectrophotometer to test the antioxidant activity of the obtained extract against DPPH free radicals at a wavelength of 517 nm, and determine the IC50 value. Routine was used as a positive control in this study. The results of three repeated studies showed that the IC50 values of the ethanol extract were 65.49 ppm, 65.76 ppm, and 65.76 ppm, respectively. Average IC50 value is 55.67 ppm.
Downloads
References
Ahmad N. 2006. Fitokimia II. Fakultas Farmasi. Universitas Muslim Indonesia
Anonim. 2008. Direktorat Obat Asli Indonesia. Badan POM RI, Jakarta
“Dalimartha, S. dan Soedibyo, M. 1999, Awet Muda Dengan Tumbuhan Obat dan Diet Suplemen.,Trubus Agriwidya, Jakarta. hal. 36-40
Fajriaty, I., Hariyanto, I. H., Andres, A., & Setyaningrum, R. (2018). Skrining Fitokimia dan Analisis Kromatografi Lapis Tipis dari Ekstrak Etanol Daun Bintangur (Calophyllum soulattri Burm. F.). Jurnal Pendidikan Informatika dan Sains, 7(1), 54-67.”
Flora E. 2011. Tanaman Obat Indonesia untuk pengobatan Herbs Medicine Herba Tanaman Obat, Indonesia
“Gandjar I.G. dan Rohman A. 2007. Kimia Farmasi Analisis. Cetakan III. Pustaka Pelajar; Yogyakarta
Pokorny, J., Yanishlieva, N., & Gordon, M. H. (Eds.). (2001). Antioxidants in food: practical applications. CRC press.
Shanmugapriya, A., Kalaiarasi, G., Kalaivani, P., Dallemer, F., & Prabhakaran, R. (2016). CT-DNA/BSA protein binding and antioxidant studies of new binuclear Pd (II) complexes and their structural characterisation. Inorganica Chimica Acta, 449, 107-118”
Winarsi, H. (2007). Antioksidan alami & radikal bebas. Pustaka Poltekkes Padang, Padang
