POTENSI EKSTRAK N-HEKSAN, DIKLOROMETANA, ETIL ASETAT, DAN ETANOL 70% JAMUR DEWA (Agaricus blazei Murill) TERHADAP SEL MCF-7
DOI:
https://doi.org/10.33759/jrki.v4i1.231Keywords:
MCF-7 cells, Extraction levels, God mushroomAbstract
God mushroom is a functional food. It has pharmacological activity. This content has activity as an anticancer. The purpose of this study was to conduct a preliminary test to obtain an active ingredient capable of having anticancer activity on MCF-7 cells. The stages of the research are stratified extraction with several solvents. Extraction using maceration. The resulting extract was identified and tested for the anticancer activity of MCF-7 cells. The test uses the TLC method. The stationary phase with silica GF254, the mobile phase is n-hexane: ethyl acetate. Anticancer activity test using the MTT method. The results of the identification of each extract contained terpenoid secondary metabolites. Anticancer activity of MCF-7 cells with IC50 extract of n-hexane 15.0923 µg/ml, dichloromethane extract 17.4213 µg/ml, ethyl acetate extract 10.9132µg/ml with strong cytotoxicity criteria, while for ethanol extract IC50 675, 1236 µg/ml.
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References
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Firenzuoli, F., Gori, L., & Lombardo, G. (2008). The Medicinal Mushroom Agaricus blazei Murrill: Review of Literature and Pharmaco-Toxicological Problems. Evidence-Based Complementary and Alternative Medicine: ECAM, 5(1), 3–15. https://doi.org/10.1093/ecam/nem007
Hirotani, M., Sai, K., Hirotani, S., & Yoshikawa, T. (2002). Blazeispirols B, C, E, and F, des-A-ergostane-type compounds, from the cultured mycelia of the fungus Agaricus blazei. Phytochemistry, 59(5), 571–577. https://doi.org/10.1016/s0031-9422(01)00445-9
Itoh, H., Ito, H., & Hibasami, H. (2008). Blazin of a new steroid isolated from Agaricus blazei Murrill (himematsutake) induces cell death and morphological change indicative of apoptotic chromatin condensation in human lung cancer LU99 and stomach cancer KATO III cells. Oncology Reports, 20(6), 1359–1361. https://doi.org/10.3892/or_00000152
Kerrigan, R.W., Callac, P., dan Parra, L.A. 2008. New and Rare Taxa in Agaricus section Bivalares (Duploannulati). Mycologia, 100: 876–892
Misgiati, M., Widyawaruyanti, A., Sukardiman, & Raharjo, S. J. (2021). Ergosterol isolated from Agaricus blazei Murill n-hexane extracts as potential anticancer MCF-7 activity. Pharmacognosy Journal, 13(2), 418–426. https://doi.org/10.5530/pj.2021.13.53
Misgiati M, & Corebima AD. (2015). Agaricus blazei Murill on the hematological parameter, random blood sugar, total cholesterol, and uric acid of Wistar rats (Sprague Dawley). Journal of Scientific Research and Studies, 2(2), 56–62. http://www.modernrespub.org/jsrs/index.htm
Onuki, R., Kawasaki, H., Baba, T., & Taira, K. (2003). Analysis of a mitochondrial apoptotic pathway using Bid-targeted ribozymes in human MCF-7 cells in the absence of a caspase-3-dependent. Antisense & Nucleic Acid Drug Development, 13(2), 75–82. https://doi.org/10.1089/108729003321629629
Peck, C.H. 1893. Report of the Botanist 1892. Annual Report on the New York State Museum of Natural History. 46: 85–149
Shimizu, T., Kawai, J., Ouchi, K., Kikuchi, H., Osima, Y., & Hidemi, R. (2016). Agarol, an ergosterol derivative from Agaricus blazei, induces caspase-independent apoptosis in human cancer cells. International Journal of Oncology, 48(4), 1670–1678. https://doi.org/10.3892/ijo.2016.3391
Takaku, T., Kimura, Y., & Okuda, H. (2001). Isolation of an antitumor compound from Agaricus blazei Murill and its mechanism of action. The Journal of Nutrition, 131(5), 1409–1413. https://doi.org/10.1093/jn/131.5.1409
Weerapreeyakul, N., Nonpunya, A., Barusrux, S., Thitimetharoch, T., & Sripanidkulchai, B. (2012). Evaluation of the anticancer potential of six herbs against a hepatoma cell line. Chinese Medicine (United Kingdom), 7. https://doi.org/10.1186/1749-8546-7-15
Wu, B., Cui, J., Zhang, C., & Li, Z. (2012). A polysaccharide from Agaricus blazei inhibits proliferation and promotes apoptosis of osteosarcoma cells. International Journal of Biological Macromolecules, 50(4), 1116–1120. https://doi.org/10.1016/j.ijbiomac.2012.02.023
Yu, C.-H., Kan, S.-F., Shu, C.-H., Lu, T.-J., Sun-Hwang, L., & Wang, P. S. (2009). Inhibitory mechanisms of Agaricus blazei Murill on the growth of prostate cancer in vitro and in vivo. The Journal of Nutritional Biochemistry, 20(10), 753–764. https://doi.org/10.1016/j.jnutbio.2008.07.004
.
Akiyamaa, & Al, E. (2011). No Title. Biochimica et BiophisicaActa (BBA), 1810(5), 519–525. ttps://www.sciencedirect.com/science/article/abs/pii/S0304416511000493?via%3Dihub
American cancer society. (2021). Breast cancer: key statistic of breast cancer. Availableat: http: //www.cancer.org/ cancer/ breastcancer/ detailedguide/ breast-cancer-key-statistics diakses pada tanggal 21 Desember 2021
Amundson, S. A., Myers, T. G., Scudiero, D., Kitada, S., Reed, J. C., & Fornace, A. J. J. (2000). An informatics approach identifying markers of chemosensitivity in human cancer cell lines. Cancer Research, 60(21), 6101–6110.
Aouali, N., Morjani, H., Trussardi, A., Soma, E., Giroux, B., & Manfait, M. (2003). Enhanced cytotoxicity and nuclear accumulation of doxorubicin-loaded nanospheres in human breast cancer MCF-7 cells expressing MRP1. Int J Oncol, 23(4), 1195–1201. https://doi.org/10.3892/ijo.23.4.1195
CCRC (Cancer Chemoprevention Research Center). MTT Method Cytotoxic Test Protocol. Yogyakarta: Faculty of Pharmacy, Gadjah Mada University, 2013
Departemen Kesehatan RI., 2019. Hari Kanker Sedunia 2019. Diakses dari https://www.kemkes.go.id/article/view/19020100003/hari-kanker-sedunia-2019.html, pada tanggal 23 November 2020
Firenzuoli, F., Gori, L., & Lombardo, G. (2008). The Medicinal Mushroom Agaricus blazei Murrill: Review of Literature and Pharmaco-Toxicological Problems. Evidence-Based Complementary and Alternative Medicine: ECAM, 5(1), 3–15. https://doi.org/10.1093/ecam/nem007
Hirotani, M., Sai, K., Hirotani, S., & Yoshikawa, T. (2002). Blazeispirols B, C, E, and F, des-A-ergostane-type compounds, from the cultured mycelia of the fungus Agaricus blazei. Phytochemistry, 59(5), 571–577. https://doi.org/10.1016/s0031-9422(01)00445-9
Itoh, H., Ito, H., & Hibasami, H. (2008). Blazin of a new steroid isolated from Agaricus blazei Murrill (himematsutake) induces cell death and morphological change indicative of apoptotic chromatin condensation in human lung cancer LU99 and stomach cancer KATO III cells. Oncology Reports, 20(6), 1359–1361. https://doi.org/10.3892/or_00000152
Kerrigan, R.W., Callac, P., dan Parra, L.A. 2008. New and Rare Taxa in Agaricus section Bivalares (Duploannulati). Mycologia, 100: 876–892
Misgiati, M., Widyawaruyanti, A., Sukardiman, & Raharjo, S. J. (2021). Ergosterol isolated from Agaricus blazei Murill n-hexane extracts as potential anticancer MCF-7 activity. Pharmacognosy Journal, 13(2), 418–426. https://doi.org/10.5530/pj.2021.13.53
Misgiati M, & Corebima AD. (2015). Agaricus blazei Murill on the hematological parameter, random blood sugar, total cholesterol, and uric acid of Wistar rats (Sprague Dawley). Journal of Scientific Research and Studies, 2(2), 56–62. http://www.modernrespub.org/jsrs/index.htm
Onuki, R., Kawasaki, H., Baba, T., & Taira, K. (2003). Analysis of a mitochondrial apoptotic pathway using Bid-targeted ribozymes in human MCF-7 cells in the absence of a caspase-3-dependent. Antisense & Nucleic Acid Drug Development, 13(2), 75–82. https://doi.org/10.1089/108729003321629629
Peck, C.H. 1893. Report of the Botanist 1892. Annual Report on the New York State Museum of Natural History. 46: 85–149
Shimizu, T., Kawai, J., Ouchi, K., Kikuchi, H., Osima, Y., & Hidemi, R. (2016). Agarol, an ergosterol derivative from Agaricus blazei, induces caspase-independent apoptosis in human cancer cells. International Journal of Oncology, 48(4), 1670–1678. https://doi.org/10.3892/ijo.2016.3391
Takaku, T., Kimura, Y., & Okuda, H. (2001). Isolation of an antitumor compound from Agaricus blazei Murill and its mechanism of action. The Journal of Nutrition, 131(5), 1409–1413. https://doi.org/10.1093/jn/131.5.1409
Weerapreeyakul, N., Nonpunya, A., Barusrux, S., Thitimetharoch, T., & Sripanidkulchai, B. (2012). Evaluation of the anticancer potential of six herbs against a hepatoma cell line. Chinese Medicine (United Kingdom), 7. https://doi.org/10.1186/1749-8546-7-15
Wu, B., Cui, J., Zhang, C., & Li, Z. (2012). A polysaccharide from Agaricus blazei inhibits proliferation and promotes apoptosis of osteosarcoma cells. International Journal of Biological Macromolecules, 50(4), 1116–1120. https://doi.org/10.1016/j.ijbiomac.2012.02.023
Yu, C.-H., Kan, S.-F., Shu, C.-H., Lu, T.-J., Sun-Hwang, L., & Wang, P. S. (2009). Inhibitory mechanisms of Agaricus blazei Murill on the growth of prostate cancer in vitro and in vivo. The Journal of Nutritional Biochemistry, 20(10), 753–764. https://doi.org/10.1016/j.jnutbio.2008.07.004
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2022-01-27
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POTENSI EKSTRAK N-HEKSAN, DIKLOROMETANA, ETIL ASETAT, DAN ETANOL 70% JAMUR DEWA (Agaricus blazei Murill) TERHADAP SEL MCF-7. (2022). Jurnal Riset Kefarmasian Indonesia, 4(1), 11-23. https://doi.org/10.33759/jrki.v4i1.231
