PENGARUH PERBEDAAN METODE EKSTRAKSI DAUN JATI (Tectona grandis L.f) TERHADAP AKTIVITAS ANTIBAKTERI PADA Escherichia coli
DOI:
https://doi.org/10.33759/jrki.v4i2.259Keywords:
Leaf of teak extract, Extration Method, AntibacterialAbstract
The use of teak is limited to wood and stems with high economic value. The part of the teak that has not been utilized optimally is the teak leaf. Teak leaves are only used traditionally as food wrappers and traditional medicines. According to several studies, Sumthong (2007) in Setyawan (2012) stated that teak leaves contain several bioactive compounds, namely tektoquinone, tannic acid, gallic acid and several other organic acids. The presence of bioactive compounds in teak leaves can also be used as an antibacterial agent. This study was conducted to determine the effect of different extraction methods maceration, percolation and microwave Microwave Assisted Extraction on the antibacterial activity of teak leaves against Escherichia coli bacteria. From the research, it can be concluded that the different extraction methods of teak (Tectona grandis L.f) leaves have a significant effect on the antibacterial activity of Escherichia coli and the microwave extraction method is effective as an antibacterial against Escherichia coli as seen from the clear zone produced which is greater than the maceration and percolation extraction methods.
Downloads
Download data is not yet available.
References
Handayani, R. (2017). Faktor-Faktor Yang Berhubungan Dengan Status Gizi Pada Anak Alfiyah, M. F., Budiretnani, D. A., & Solikin, N. 1978. Uji Ekstrak Etanol Daun jati (Tectona grandis L.f) ( Tectona grandis ) sebagai Bahan Pengawet Alami Daging Sapi. Prosiding Semnas Hayati JV, Hal. 94–102.
Bayer 1. Bayer HealthCare BPC. (Ciprofloxacin Hydrochloride) Tabelts. Cipro. 2009;31. HealthCare, B. P. C. 2009. (ciprofloxacin hydrochloride) TABELTS. Cipro, 31.
Belwal, T., Ezzat, S. M., Rastrelli, L., Bhatt, I. D., Daglia, M., Baldi, A., Devkota, H. P., Orhan, I. E., Patra, J. K., Das, G., Anandharamakrishnan, C., Gomez-Gomez, L., Nabavi, S. F., Nabavi, S. M., and Atanasov, A. G. A., 2018. Critical Analysis of Extraction Techniques Used for Botanicals: Trends, Priorities, Industrial Uses and Optimization Strategies. Trac- Trends Analytical Chemistry 100, 82–102. doi: 10.1016/j.trac.2017.12.018.
Camel, V., 2000. MAE (Microwave Assisted Extraction)-assisted solvent extraction of environmental samples. Trac- Trends Analytical Chemistry 19, 229–248. doi: 10.1016/S0165-9936(99)00185-5.
Chastelyna, A. J. 2016. Uji Aktivitas Antibakteri Sabun Cair Ekstrak Daun jati (Tectona grandis L.f) (Tectona Grandis Lf) sebagai Antibakteri Terhadap Staphylococcus aureus dan Escherichia coli. Skripsi. Semarang : Universitas Negeri Semarang
Crawford, J. A., Blank, T. E., & Kaper, J. B. 2002. The LEE-Encoded Type III Secretion System in EPEC and EHEC: Assembly, Function, and Regulation. Escherichia Coli, Vol. 4(1), Hal. 337–359.
DEPKES RI. 1979. Farmakope Indonesia edisi III. Jakarta : Departemen Kesehatan Republik Indonesia
Devi Novia, Agung G.S., Camelia ZA. 2020. Pengaruh Pemberian Infusa Daun jati (Tectona grandis L.f) (Tectona grandis L.S) Terhadap Waktu Kematian Cacing Ascaridia galli Sp Secara In Vitro. Jurnal Ilmiah Farmasi, Vol. 7(1), Hal. 92027.
Esther, Fiammeta. 2017. Karakteristik Senyawa Antibakteri Ekstrak Daun jati (Tectona grandis L.f) (Tectona grandis L.). Tesis Sarjana. Tangerang : Universitas Pelita Harapan.
Fitriana. 2010. Pengaruh Metode Ektsraksi Terhadap Aktivitas Antimikroba Ekstrak Metanol Daun jati (Tectona grandis L.f) (Tectona grandis L.F). Skripsi. Makassar : Universitas Islam Negeri Alauddin
Ganiswarna, S.G. 1995. Farmakologi dan Terapi edisi V. Jakarta : Universitas Indonesia
Greenwood, D., Finch, R., Davey, P. and Wilcox, M. 1995. Antibiotics, Susceptibility (Sensitivity) Test Antimicrobial and Chemoterapy. United State of America: Mc Graw Hill Company
Hangat, S., Asuhan, P., Gerontik, K., Hangat, S., Asuhan, P., & Gerontik, K. 2019. Pertumbuhan Bakteri Selama Penyimpanan Daging Sapi Dengan Pengemas Daun jati (Tectona grandis L.f) (Tectona grandis) Dan Daun Pisang (Musa paradisiaca). Skripsi. Surakarta : Institut Teknologi Sains dan Kesehatan PKU Muhammadiyah
Hasnaeni, H., & Wisdawati, W. 2019. Pengaruh metode ekstraksi terhadap rendemen dan kadar fenolik ekstrak tanaman Kayu Beta-beta (Lunasia amara Blanco). Jurnal Farmasi Galenika (Galenika Journal of Pharmacy)(e-Journal), 5(2), 175-182.
Ifnawati. 2013. Pengaruh Enzim Kitinase Kasar dari Bakteri Pseudomonas pseudomallei dan Klebsiella ozaenae Terhadap Pertumbuhan, Morfologi, dan Kadar N-Asetilglukosamin Fusarium oxysporum. Skripsi. Malang : Universitas Islam Negeri Maulana Malik Ibrahim.
Jain, T., V. Jain, R. Pandey, A. Vyas dan S.Shukla. 2009. Microwave assisted extraction for phytoconstituents – an overview. Asian Journal Research Chemistry 2: 19-25.
Langat, M. K. 2011. Chemical Constituents of East European Forest Species. Book of Extended Extracts, Kenya
Mandal, V., Mohan, Y., Hemalatha, S. 2007. Microwave Assisted Extraction-An Innovative and Promosing Extraction Tool For Medicinal Plant Research.Pharmaconosy Reviews, 1: 7-18.
Mthiyane, M. N., & Hugo, A. 2019. Isolasi Dan Karakterisasi Fungi Endofit Dari Kulit Buah Manggis (Garcinia mangostana L) Sebagai Antimikroba Terhadap Candida albicans, Staphylococcus aureus Dan Escherichia coli. Jurnal Tjyybjb.Ac.Cn, Vol. 3(2252), Hal. 58–66.
Ngajow, M., Abidjulu, J., & Kamu, V. S. 2013. Antibacterial Effect of Matoa Stem (Pometia pinnata) peels Extract to Staphylococcus aureus Bacteria In Vitro. Jurnal MIPA UNSRAT, Vol. 2(2), Hal. 128–132.
Nidavani, R. B., & Am, M. 2014. Teak (Tectona grandis Linn.): A renowned timber plant with potential medicinal values. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 6(1), Hal. 48–54.
Noventi, W. R.-4272-2-P. pdfa., & Carolia, N. 2016. Potensi Ekstrak Daun Sirih Hijau ( Piper betle L.) sebagai Alternatif Terapi Acne vulgaris. Jurnal Studi Pendidikan Dokter Fakultas Kedokteran Universitas Lampung, Vol. 5(1), Hal. 140.
Nuryastuti, T., Van Der Mei, H. C., Busscher, H. J., Iravati, S., Aman, A. T., & Krom, B. P. 2009. Effect of cinnamon oil on icaA expression and biofilm formation by Staphylococcus epidermidis. Jurnal Applied and Environmental Microbiology, Vol. 75(21), Hal. 6850–6855.
Oktovia, D. H. 2017. Uji Aktivitas Bakteri Menggunakan Metode Cakram Disk (Kirby Bauer). Laporan Penelitian. Banjarmasin : Politeknik Kesehatan Kementrian Kesehatan
Paturusi, A. A. E., Nurafianty, Rusli, & Rahim, A. 2014. Isolasi dan Identifikasi Senyawa Antibakteri Ekstrak N-Heksan Daun jati (Tectona grandis L.f) (Tectona grandis L.F). Jurnal Fik Uinam, Vol. 2(1), Hal. 18–23.
Prayoga, E. 2013. Perbandingan Efek Ekstrak Daun sirih hijau (Piper betle L.) dengan metode difusi disk dan sumuran terhadap pertumbuhan bakteri Staphylococcus aureus. Journal Foundations of Physics, Vol. 34(3), Hal. 361–403.
Routray, W., and Orsat, V., 2012. MAE (Microwave Assisted Extraction)-Assisted Extraction of Flavonoid: A Review. Food Bioprocess Technology 5, 409-424. doi: 10.1007/s11947-011-0573-z.
Rijayanti, R. P., Luliana, S., & Trianto, H. F. 2014. In vitro Antibacterial Activity test Of Ethanol Extracts Bacang mango (Mangifera foetida L.) Leaves Against Staphylococcus aureus. Naskah Publikasi Universitas Tanjungpura, Vol. 1(1), Hal. 10–12.
Setyawan. 2012. Uji Aktivitas Antibakteri Ekstrak Kasar Daun jati (Tectona grandis L.f) (Tectona grandis) Metode MAE (Microwave Assisted Extraction) Assisted Extraction terhadap Escherichia coli dan Staphylococcus aureus (Kajian Rasio Sampel : Pelarut dan Jumlah). Tesis Sarjana. Malang : Universitas Brawijaya
Siradjudin, Mutmainnah. 2014. Ekstraksi Sampel Mali-Mali (Leea indica L.). Laporan Penelitian. Jakarta : Universitas Muhammadiyah
Spigno, G., and De Faveri, D.M., 2009. Microwave-assisted extraction of tea phenols: A phenomenological study. Journal of Food Engineering 93, 210-217. doi: 10.1016/j.jfoodeng.2009.01.006.
Sumampouw, O. J. 2018. Uji Sensivitas Antibiotik Terhadap Bakteri Escherichia coli Penyebab Diare Balita Di Kota Manado ( The Sensitivity Test of Antibiotics to Escherichia coli was Caused The Diarhhea on Underfive Children in Manado City). Journal of Current Pharmaceutical Sciences, Vol. 2(1), Hal. 105.
Sutiknowati, L. I. 2016. Bioindikator Pencemar Bakteri Escherichia coli. Journal Oseana, Vol. 41(4), Hal. 63–71. oseanografi.lipi.go.id
Syamsuni, H.A. 2016. Ilmu Resep. Jakarta : Buku Kedokteran EGC
Tumbel, Maria. 2009. Uji Daya Hambat Ekstrak Metanol Daun jati (Tectona grandis L.f) (Guazuma ulmifolia, Lamk) terhadap Pertumbuhan Eschericia coli. Jurnal Chemica, Vol. 2(9), Hal. 85-91.
Utami, A.D. 2017. Ekstraksi. Laporan Penelitian. Jakarta : Universitas Muhammadiyah
Yazid, M., Bastianudin, A., Saputra, T., Triatmojo, S., Pertiwiningrum, A., Perdana, D. A., Ebrianto, A. L., Sari, T. I., Sumatera, K., Darmanto, A., Soeparman, S., Widhiyanuriawan, D., Khaerunnisa, G., Rahmawati, I., Putri, A., Salahuddin, N. S., Gumay, M. G., Wisudawati, N., Gustiar, F.,Rahardjo, S. 2014. Total Jumlah Bakteri Pada Daging Sapi Segar Yang Dibungkus Daun jati (Tectona grandis L.f) Dengan Variasi Lama Penyimpanan. Jurnal Teknologi Kimia Dan Industri, Vol. 2(1), Hal. 1–7.
Zhu, C., Harel, J., Jacques, M., Desautels, C., Donnenberg, M. S., Beaudry, M., & Fairbrother, J. M. (1994). Virulence properties and attaching-effacing activity of Escherichia coli O45 from swine postweaning diarrhea. Journal Infection and Immunity, Vol. 62(10), Hal. 4153–4159.
Bayer 1. Bayer HealthCare BPC. (Ciprofloxacin Hydrochloride) Tabelts. Cipro. 2009;31. HealthCare, B. P. C. 2009. (ciprofloxacin hydrochloride) TABELTS. Cipro, 31.
Belwal, T., Ezzat, S. M., Rastrelli, L., Bhatt, I. D., Daglia, M., Baldi, A., Devkota, H. P., Orhan, I. E., Patra, J. K., Das, G., Anandharamakrishnan, C., Gomez-Gomez, L., Nabavi, S. F., Nabavi, S. M., and Atanasov, A. G. A., 2018. Critical Analysis of Extraction Techniques Used for Botanicals: Trends, Priorities, Industrial Uses and Optimization Strategies. Trac- Trends Analytical Chemistry 100, 82–102. doi: 10.1016/j.trac.2017.12.018.
Camel, V., 2000. MAE (Microwave Assisted Extraction)-assisted solvent extraction of environmental samples. Trac- Trends Analytical Chemistry 19, 229–248. doi: 10.1016/S0165-9936(99)00185-5.
Chastelyna, A. J. 2016. Uji Aktivitas Antibakteri Sabun Cair Ekstrak Daun jati (Tectona grandis L.f) (Tectona Grandis Lf) sebagai Antibakteri Terhadap Staphylococcus aureus dan Escherichia coli. Skripsi. Semarang : Universitas Negeri Semarang
Crawford, J. A., Blank, T. E., & Kaper, J. B. 2002. The LEE-Encoded Type III Secretion System in EPEC and EHEC: Assembly, Function, and Regulation. Escherichia Coli, Vol. 4(1), Hal. 337–359.
DEPKES RI. 1979. Farmakope Indonesia edisi III. Jakarta : Departemen Kesehatan Republik Indonesia
Devi Novia, Agung G.S., Camelia ZA. 2020. Pengaruh Pemberian Infusa Daun jati (Tectona grandis L.f) (Tectona grandis L.S) Terhadap Waktu Kematian Cacing Ascaridia galli Sp Secara In Vitro. Jurnal Ilmiah Farmasi, Vol. 7(1), Hal. 92027.
Esther, Fiammeta. 2017. Karakteristik Senyawa Antibakteri Ekstrak Daun jati (Tectona grandis L.f) (Tectona grandis L.). Tesis Sarjana. Tangerang : Universitas Pelita Harapan.
Fitriana. 2010. Pengaruh Metode Ektsraksi Terhadap Aktivitas Antimikroba Ekstrak Metanol Daun jati (Tectona grandis L.f) (Tectona grandis L.F). Skripsi. Makassar : Universitas Islam Negeri Alauddin
Ganiswarna, S.G. 1995. Farmakologi dan Terapi edisi V. Jakarta : Universitas Indonesia
Greenwood, D., Finch, R., Davey, P. and Wilcox, M. 1995. Antibiotics, Susceptibility (Sensitivity) Test Antimicrobial and Chemoterapy. United State of America: Mc Graw Hill Company
Hangat, S., Asuhan, P., Gerontik, K., Hangat, S., Asuhan, P., & Gerontik, K. 2019. Pertumbuhan Bakteri Selama Penyimpanan Daging Sapi Dengan Pengemas Daun jati (Tectona grandis L.f) (Tectona grandis) Dan Daun Pisang (Musa paradisiaca). Skripsi. Surakarta : Institut Teknologi Sains dan Kesehatan PKU Muhammadiyah
Hasnaeni, H., & Wisdawati, W. 2019. Pengaruh metode ekstraksi terhadap rendemen dan kadar fenolik ekstrak tanaman Kayu Beta-beta (Lunasia amara Blanco). Jurnal Farmasi Galenika (Galenika Journal of Pharmacy)(e-Journal), 5(2), 175-182.
Ifnawati. 2013. Pengaruh Enzim Kitinase Kasar dari Bakteri Pseudomonas pseudomallei dan Klebsiella ozaenae Terhadap Pertumbuhan, Morfologi, dan Kadar N-Asetilglukosamin Fusarium oxysporum. Skripsi. Malang : Universitas Islam Negeri Maulana Malik Ibrahim.
Jain, T., V. Jain, R. Pandey, A. Vyas dan S.Shukla. 2009. Microwave assisted extraction for phytoconstituents – an overview. Asian Journal Research Chemistry 2: 19-25.
Langat, M. K. 2011. Chemical Constituents of East European Forest Species. Book of Extended Extracts, Kenya
Mandal, V., Mohan, Y., Hemalatha, S. 2007. Microwave Assisted Extraction-An Innovative and Promosing Extraction Tool For Medicinal Plant Research.Pharmaconosy Reviews, 1: 7-18.
Mthiyane, M. N., & Hugo, A. 2019. Isolasi Dan Karakterisasi Fungi Endofit Dari Kulit Buah Manggis (Garcinia mangostana L) Sebagai Antimikroba Terhadap Candida albicans, Staphylococcus aureus Dan Escherichia coli. Jurnal Tjyybjb.Ac.Cn, Vol. 3(2252), Hal. 58–66.
Ngajow, M., Abidjulu, J., & Kamu, V. S. 2013. Antibacterial Effect of Matoa Stem (Pometia pinnata) peels Extract to Staphylococcus aureus Bacteria In Vitro. Jurnal MIPA UNSRAT, Vol. 2(2), Hal. 128–132.
Nidavani, R. B., & Am, M. 2014. Teak (Tectona grandis Linn.): A renowned timber plant with potential medicinal values. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 6(1), Hal. 48–54.
Noventi, W. R.-4272-2-P. pdfa., & Carolia, N. 2016. Potensi Ekstrak Daun Sirih Hijau ( Piper betle L.) sebagai Alternatif Terapi Acne vulgaris. Jurnal Studi Pendidikan Dokter Fakultas Kedokteran Universitas Lampung, Vol. 5(1), Hal. 140.
Nuryastuti, T., Van Der Mei, H. C., Busscher, H. J., Iravati, S., Aman, A. T., & Krom, B. P. 2009. Effect of cinnamon oil on icaA expression and biofilm formation by Staphylococcus epidermidis. Jurnal Applied and Environmental Microbiology, Vol. 75(21), Hal. 6850–6855.
Oktovia, D. H. 2017. Uji Aktivitas Bakteri Menggunakan Metode Cakram Disk (Kirby Bauer). Laporan Penelitian. Banjarmasin : Politeknik Kesehatan Kementrian Kesehatan
Paturusi, A. A. E., Nurafianty, Rusli, & Rahim, A. 2014. Isolasi dan Identifikasi Senyawa Antibakteri Ekstrak N-Heksan Daun jati (Tectona grandis L.f) (Tectona grandis L.F). Jurnal Fik Uinam, Vol. 2(1), Hal. 18–23.
Prayoga, E. 2013. Perbandingan Efek Ekstrak Daun sirih hijau (Piper betle L.) dengan metode difusi disk dan sumuran terhadap pertumbuhan bakteri Staphylococcus aureus. Journal Foundations of Physics, Vol. 34(3), Hal. 361–403.
Routray, W., and Orsat, V., 2012. MAE (Microwave Assisted Extraction)-Assisted Extraction of Flavonoid: A Review. Food Bioprocess Technology 5, 409-424. doi: 10.1007/s11947-011-0573-z.
Rijayanti, R. P., Luliana, S., & Trianto, H. F. 2014. In vitro Antibacterial Activity test Of Ethanol Extracts Bacang mango (Mangifera foetida L.) Leaves Against Staphylococcus aureus. Naskah Publikasi Universitas Tanjungpura, Vol. 1(1), Hal. 10–12.
Setyawan. 2012. Uji Aktivitas Antibakteri Ekstrak Kasar Daun jati (Tectona grandis L.f) (Tectona grandis) Metode MAE (Microwave Assisted Extraction) Assisted Extraction terhadap Escherichia coli dan Staphylococcus aureus (Kajian Rasio Sampel : Pelarut dan Jumlah). Tesis Sarjana. Malang : Universitas Brawijaya
Siradjudin, Mutmainnah. 2014. Ekstraksi Sampel Mali-Mali (Leea indica L.). Laporan Penelitian. Jakarta : Universitas Muhammadiyah
Spigno, G., and De Faveri, D.M., 2009. Microwave-assisted extraction of tea phenols: A phenomenological study. Journal of Food Engineering 93, 210-217. doi: 10.1016/j.jfoodeng.2009.01.006.
Sumampouw, O. J. 2018. Uji Sensivitas Antibiotik Terhadap Bakteri Escherichia coli Penyebab Diare Balita Di Kota Manado ( The Sensitivity Test of Antibiotics to Escherichia coli was Caused The Diarhhea on Underfive Children in Manado City). Journal of Current Pharmaceutical Sciences, Vol. 2(1), Hal. 105.
Sutiknowati, L. I. 2016. Bioindikator Pencemar Bakteri Escherichia coli. Journal Oseana, Vol. 41(4), Hal. 63–71. oseanografi.lipi.go.id
Syamsuni, H.A. 2016. Ilmu Resep. Jakarta : Buku Kedokteran EGC
Tumbel, Maria. 2009. Uji Daya Hambat Ekstrak Metanol Daun jati (Tectona grandis L.f) (Guazuma ulmifolia, Lamk) terhadap Pertumbuhan Eschericia coli. Jurnal Chemica, Vol. 2(9), Hal. 85-91.
Utami, A.D. 2017. Ekstraksi. Laporan Penelitian. Jakarta : Universitas Muhammadiyah
Yazid, M., Bastianudin, A., Saputra, T., Triatmojo, S., Pertiwiningrum, A., Perdana, D. A., Ebrianto, A. L., Sari, T. I., Sumatera, K., Darmanto, A., Soeparman, S., Widhiyanuriawan, D., Khaerunnisa, G., Rahmawati, I., Putri, A., Salahuddin, N. S., Gumay, M. G., Wisudawati, N., Gustiar, F.,Rahardjo, S. 2014. Total Jumlah Bakteri Pada Daging Sapi Segar Yang Dibungkus Daun jati (Tectona grandis L.f) Dengan Variasi Lama Penyimpanan. Jurnal Teknologi Kimia Dan Industri, Vol. 2(1), Hal. 1–7.
Zhu, C., Harel, J., Jacques, M., Desautels, C., Donnenberg, M. S., Beaudry, M., & Fairbrother, J. M. (1994). Virulence properties and attaching-effacing activity of Escherichia coli O45 from swine postweaning diarrhea. Journal Infection and Immunity, Vol. 62(10), Hal. 4153–4159.
Downloads
Published
2022-05-31
Issue
Section
Articles
How to Cite
PENGARUH PERBEDAAN METODE EKSTRAKSI DAUN JATI (Tectona grandis L.f) TERHADAP AKTIVITAS ANTIBAKTERI PADA Escherichia coli. (2022). Jurnal Riset Kefarmasian Indonesia, 4(2), 156-173. https://doi.org/10.33759/jrki.v4i2.259
