SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL RIMPANG KUNYIT HITAM (Curcuma caesia Roxb.) ASAL LUWU UTARA DENGAN METODE DPPH
DOI:
https://doi.org/10.33759/jrki.v5i3.397Keywords:
Kata kunci: Antioksidan; Curcuma caesia Roxb.; DPPH; Skrining FitokimiaAbstract
The ethanol extract of black turmeric (Curcuma caesia Roxb.) contains flavonoids compounds which are thought to have antioxidant activity.The purpose of this study was to determine the class of chemical compounds and determine the IC50 value of the ethanol extract of black turmeric (Curcuma caesia Roxb.)rhizome from North Luwu using the DPPH method. The research method included extraction by maceration using 70% ethanol solvent. Phytochemical screening using specific reagents and antioxidant activity testing using the DPPH method using a visible spectrophotometer at a wavelength of 515 nm. The results of the phytochemical screening showed that the ethanol extract of black turmeric rhizome contained flavonoids, alkaloids, tannins and triterpenoids. The results of the antioxidant activity test showed that the ethanol extract of black turmeric (Curcuma caesia Roxb.) rhizome had an IC50 value of 108.8304 ± 1.24 μg/mL and ascorbic acid of 2.39 ± 0.04 μg/mL.
Downloads
References
Achmad, Klagus Roni. 1986. Kimia Organik. Jakarta: Karnunika.
Arista Wahyu Ningsih, Iif Hanifa Nurrosyidah, and A’Yunil Hisbiyah. 2018. “Pengaruh Perbedaan Metode Ekstraksi Rimpang Kunyit (Curcuma Domestica) Terhadap Rendemen Dan Skrining Fitokimia.” Journal of Pharmaceutical-Care Anwar Medika 2(2):49–57. doi: 10.36932/jpcam.v2i2.27.
Baghel, Satyendra Singh, Rajendra Singh Baghel, Kshamashil Sharma, and Indu Sikarwar. 2013. “Aktivitas Farmakologi Curcuma Caesia.” doi: 10.4103/0973-8258.111590.
Depkes RI. 1985. Cara Pembuatan Simplisia. Jakarta: Departemen Kesehatan Republik Indonesia.
Depkes RI. 1995. Farmakope Indonesia Edisi IV. Jakarta: Departemen Kesehatan Republik Indonesia.
Devi, Heisanam Pushparani, P. B. Mazumder, and Laishram Priyadarshini Devi. 2015. “Antioxidant and Antimutagenic Activity of Curcuma Caesia Roxb. Rhizome Extracts.” Toxicology Reports 2:423–28. doi: 10.1016/j.toxrep.2014.12.018.
Direktorat Jendral Pengawas Obat dan makanan. 2017. Farmakope Herbal Indonesia Edisi II. Jakarta: Departemen Kesehatan Republik Indonesia.
Endarini. L. H. 2016. Farmakognosi Dan Fitokimia. Jakarta: Badan Pengembangan Dan Pemberdayaan Sumber Daya Manusia Kesehatan.
Erlidawati, and Safrida. 2018. Potensi Antioksidan Sebagai Antidiabetes : Buku Untuk Mahasiswa. Aceh: Syiah Kuala University Press.
Harborne, B. 1987. Methods in Plant Biochemistry. New York: Plenum Press.
Hart Brown, William, Brent Iverson, Eric Anslyn, and Christopher Foote. 1990. “Chimica Organica.” Organic Letters.
Irianti, T. T., and S. Nuranto. 2021. Antioksidan Dan Kesehatan. Yoggyakarta: UGM PRESS.
Julianto, Tatang Shabur. 2018. Fitokimia Tinjauan Metabolit Sekunder Dan Skrining Fitokimia. Yogyakarta: Universitas Islam Indonesia.
Karmakar, Indrajit, Narayan Dolai, and Pathik Saha. 2011. “Aktivitas Pemulungan Rimpang Curcuma Caesia Terhadap Oksigen Reaktif Dan Spesies Nitrogen.” 221–28. doi: 10.1007/s13596-011-0030-6.
McMurry, John, and R. C. Fay. 2004. “Organic Chemistry.” Pearson Education Interntional.
Miroslav, Vecera, and Jiri Gasparic. 1971. Detection And Identification Of Organic Compounds. New York: Planung Publishing Corporation and SNTC Publishert Of Technical Literatur.
Molyneux, Philip. 2004. “Penggunaan Radikal Bebas Difenilpikril Hidrazil ( DPPH ) Yang Stabil Untuk Pendugaan Aktivitas Antioksidan.” Songklanakarin J. Sci Teknologi 26(2):211–19.
Mulyono. H. 2005. Membuat Reagen Kimia Di Laboratorium. Jakarta: Bumi Aksara.
Nugrahani, Rizki, and Yayuk Andayani. 2016. “Skrining Fitokimia Dari Ekstrak Buah Buncis Phaseeolus Vulgaris Dalam Sediaan Serbuk.” Jurnal Penelitian Pendidikan Ipa 2(1).
Pakkirisamy, Muthukumaran, Suresh Kumar Kalakandan, and Karthikeyen Ravichandran. 2014. “Phytochemical Screening, GC-MS, FT-IR Analysis of Methanolic Extract of Curcuma Caesia Roxb (Black Turmeric).” Pharmacognosy Journal 9(6):952–56. doi: 10.5530/pj.2017.6.149.
Pathan, Aslam. 2017. “Phytochemical Investigations of Indigenous Herb: Curcuma Caesia Rhizomes.” NeuroPharmac Journal 2(2):41–46. doi: 10.37881/1.221.
Paw, Manabi, Roktim Gogoi, Neelav Sarma, Sudin K. Pandey, Angana Borah, Twahira Begum, and Mohan Lal. 2019. “ Study of Anti-Oxidant, Anti-Inflammatory, Genotoxicity, and Antimicrobial Activities and Analysis of Different Constituents Found in Rhizome Essential Oil of Curcuma Caesia Roxb., Collected from North East India .” Current Pharmaceutical Biotechnology 21(5):403–13. doi: 10.2174/1389201020666191118121609.
Sayuti, Kesuma, and Rina Yenrina. 2015. Alami Dan Sintetik (1 Ed). Padang: Andalas University Press.
Sdedi, Desire Janetha, Hanggara Arifian, and Laode Rijai. 2016. “Uji Aktivitas Antibakteri Ekstrak Rimpang Kunyit Hitam (Curcuma Caesia ROXB. ).” Prosiding Seminar Nasiona Kefarmasian Ke-420–21.
Sudewo, B., and Z. Simatur. 2011. Basmi Kanker Dengan Herbal. Jakarta: VisiMedia.
Sufardi. 2020. “Pertumbuhan Tanaman.” Researchgate (May):1–26.
Svehla, G. 1990. Textbook of Macro and Semimicro Qualitative Inorganic Analysis. New York: United States Of America.
Yadav, Mamta, and K. Kalyan Saravanan. 2019. “Phytochemical Analysis and Antioxidant Potential of Rhizome Extracts of Curcuma Amada Roxb and Curcuma Caesia Roxb.” Journal of Drug Delivery and Therapeutics 9(5):123–26. doi: 10.22270/jddt.v9i5.3609.
Youngson, R. 2005. Antioksidan: Manfaat Vitamin C Dan E Bagi Kesehatan. Jakarta: Egc Arcan.
Yunanto, Ari, Bambang Setiawan, and Eko Suhartono. 2009. Kapita Selekta Biokimia Peran Radikal Bebas Pada Intoksikasi Dan Patobiologi Penyakit. Banjarmasin: Penerbit Pustaka Benua.
Yuslianti, E. R. 2018. Pengantar Radikal Bebas Dan Antioksidan. Yogyakarta: CV Budi Utama.
Downloads
Published
Issue
Section
License
Copyright (c) 2023 Jurnal Riset Kefarmasian Indonesia
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
