PENETAPAN KADAR ALKALOID TOTAL PADA EKSTRAK N-HEKSAN DAN ETANOL BIJI KETUMBAR (Coriandrum sativum) MENGGUNAKAN SPEKTROFOTOMETER UV-Vis
DOI:
https://doi.org/10.33759/jrki.v4i3.268Keywords:
Alkaloids, Berberine, Bromocresol green (BCG),Abstract
Coriander (Coriandrum sativum) is one of the medicinal plants which is known to contain secondary metabolites such as flavonoids, tannins, terpenoids, saponins, steroids, and alkaloids. Alkaloids have therapeutic effects as antimalarials and cancer. Research in determining the alkaloids in coriander was carried out by separating the alkaloids from other substances using the soxhlet method extraction with two solvents ethanol and n-hexane. The total alkaloid content is determined using a UV-VIS spectrophotometer at wavelength 351,5 nm. The reaction of alkaloids with Bromocresol green (BCG) is obtained to form a yellow product. The use of berberine standard was obtained the total alkaloid content of ethanol and n-hexane extract in coriander seed (Coriandrum sativum) were 0.524% and 0.583% respectively, and the ratio of total alkaloid content of ethanol extract was smaller than that of n-hexane extract.
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References
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Altun, M. L. (2002). HPLC method for the analysis of paracetamol, caffeine and dipyrone. Turkish Journal of Chemistry, 26(4), 521–528.
Chen, Q.-C., & Wang, J. (2001). Simultaneous determination of artificial sweeteners, preservatives, caffeine, theobromine and theophylline in food and pharmaceutical preparations by ion chromatography. Journal of Chromatography A, 937(1–2), 57–64.
Dayanti, r. (2012). Aktivitas antioksidan ekstrak metanol bagian batang tumbuhan paku nephrolepis radicans (burm.) Kuhn (activities antioxidant methanol plant extract nails nephrolepis radicans (burm.) Kuhn). Unesa journal of chemistry, 1(1).
Depkes, R. I. (2000). Parameter standar umum ekstrak tumbuhan obat. Jakarta: Departemen Kesehatan Republik Indonesia, 3–30.
Handayani, P. A., & Juniarti, E. R. (2012). Ekstraksi minyak ketumbar (coriander oil) dengan pelarut etanol dan N-heksana. Jurnal Bahan Alam Terbarukan, 1(1).
Hartati, S. (2010). The intergeneric crossing of Phalaenopsis sp. and Vanda tricolor. Journal of Biotechnology and Biodiversity, 1(1), 32–36.
Jordan, C. H., Logel, C., Spencer, S. J., Zanna, M. P., Wood, J. V, & Holmes, J. G. (2013). Responsive low self-esteem: Low explicit self-esteem, implicit self-esteem, and reactions to performance outcomes. Journal of Social and Clinical Psychology, 32(7), 703–732.
Katayama, M., & Taniguchi, H. (1989). Fluorometric reactions of purines and determination of caffeine. Talanta, 36(12), 1171–1175.
Khopkar, S. . (2003). konsep dasar kimia analitik. UI pres.
Nathenial, S., Fatima, A., Fatima, R., & Ijaz, N. (2019). Phytochemical study of acetone solvent extract of Coriander sativum. Journal of Pharmacognosy and Phytochemstry, 8(6), 136–140.
NURYANTI, S. (1993). Identifikasi senyawa alkaloid dari batang kayu kuning (Arcangelisia Flava Merr). Universitas Gadjah Mada.
Pagliarussi, R. S., Freitas, L. A. P., & Bastos, J. K. (2002). A quantitative method for the analysis of xanthine alkaloids in Paullinia cupana (guarana) by capillary column gas chromatography. Journal of Separation Science, 25(5‐6), 371–374.
Patel, R. K., Patel, J. B., & Trivedi, P. D. (2015). Spectrophotometric method for the estimation of total alkaloids in the Tinospora cordifolia M. and its herbal formulations. International Journal of Pharmacy and Pharmaceutical Sciences, 7(10), 249–251.
Perwita, F. A. (2011). Teknologi ekstraksi daun ungu (Graptophyllum pictum) dalam ethanol 70% dengan metode perkolasi.
Pfoze, N. L., Myrboh, B., Kumar, Y., & Rohman, M. R. (2014). Isolation of protoberberine alkaloids from stem bark of Mahonia manipurensis Takeda using RP-HPLC. Journal of Medicinal Plants, 2(2), 48–57.
Pradhan, L., Nabzdyk, C., Andersen, N. D., LoGerfo, F. W., & Veves, A. (2009). Inflammation and neuropeptides: the connection in diabetic wound healing. Expert Reviews in Molecular Medicine, 11.
Qing-qin, X., Ming, D. L., Wang, J. P., & Bai, A. H. (2002). Direct determination of caffeine and theophylline by gas chromatography. Fenxi Kexue Xuebao, 18, 520–525.
Rifai, M. A. (1976). Sendi-sendi botani sistematika. LBN-LIPI. Bogor.
Robinson, T. (1995). Kandungan Senyawa Organik Tumbuhan Tinggi (Diterjemahkan oleh Prof. Dr. Kosasih Padmawinata (ed.)). ITB.
Shamsa, F., Monsef, H., Ghamooshi, R., & Verdian-rizi, M. (2008). Spectrophotometric determination of total alkaloids in some Iranian medicinal plants. Thai J Pharm Sci, 32, 17–20.
Shriner, R. L., Hermann, C. K. F., Morrill, T. C., Curtin, D. Y., & Fuson, R. C. (2003). The systematic identification of organic compounds. John Wiley & Sons.
Tabasum, S., Khare, S., & Jain, K. (2016). Spectrophotometric quantification of total phenolic, flavonoid, and alkaloid contents of Abrus precatorius L. seeds. Asian J Pharm Clin Res, 9(2), 371–374.
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Published
2022-09-30
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PENETAPAN KADAR ALKALOID TOTAL PADA EKSTRAK N-HEKSAN DAN ETANOL BIJI KETUMBAR (Coriandrum sativum) MENGGUNAKAN SPEKTROFOTOMETER UV-Vis. (2022). Jurnal Riset Kefarmasian Indonesia, 4(3), 353-365. https://doi.org/10.33759/jrki.v4i3.268
