UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN TABAT BARITO (Ficus deltoidea) TERHADAP BAKTERI Bacillus substillis DENGAN TINGKATAN POLARITAS PELARUT
DOI:
https://doi.org/10.33759/jrki.v4i1.229Keywords:
Bacillus substillis, Antibacterial, Tabat baritoAbstract
Tabat barito leaf (Ficus deltoidea) is a single leaf of a sole form, the leaf has round tip with a flat edge and a pointed base. These leaves have a smooth surface, coloured green above and brownish yellow beneath. This study aims to determine the antibacterial activity of polar, semi-polar and non-polar compounds contained in Tabat barito leaf extract extracted with solvents of different polarity. The highest bacterial inhibition was also measured against Bacillus substillis which is a gram-positive bacterial. The maceration method uses 3 levels of solvents with different polarities, started with a non-polar solvent (n-hexan), a semi-polar solvent (ethyl acetate) and finally macerated with a polar solvent (methanol). The bacterial activity test was done with diffusion method. The results showed that the Tabat Barito leaf extract contained antibacterial compounds that were non-polar, semi-polar and polar. The highest inhibition of the hexane extract at concentration 15% with a clear zone diameter of 22.33 mm in very strong inhibition category against Bacillus substilis bacteria
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References
Cowan, M. M. (1999). Plant products as antimicrobial agents. Clinical Microbiology Reviews, 12(4), 564–582. https://doi.org/10.1128/cmr.12.4.564
DAVIS, S. (1971). Disc Plate Method of Microbiological Antibiotic Assay. 22(4), 659–665.
El Jannah, S. M., Rahayu, C., Zuraida, Prasetio, R., & Sugiarto, R. I. (2017). PRELIMINARY RESEARCH: IDENTIFICATION OF MICROORGANISM IN THE WAITING ROOM ON PUBLIC TRANSPORTATION FACILITIES, DKI JAKARTA. 8843, 9–15.
Fitriah, F., Mappiratu, M., & Prismawiryanti, P. (2017). UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN TANAMAN JOHAR (Cassia siamea Lamk.) DARI BEBERAPA TINGKAT KEPOLARAN PELARUT. Kovalen, 3(3), 242. https://doi.org/10.22487/j24775398.2017.v3.i3.9333
Isnaini, M., Dewi, E. N., & Rianingsih, L. (2018). KAJIAN POTENSI EKSTRAK ANGGUR LAUT (Caulerpa racemosa) SEBAGAI ANTIBAKTERI TERHADAP BAKTERI Escherichia coli DAN Staphylococcus aureus. J. Peng. & Biotek. Hasil Pi., 7(1), 7–14.
Jawetz, Melinick, & Aldeberg. (2008). Mikrobiologi Iftdokteran. 23, 251–257.
Loekitowati Poedji,Fahma, A. A. (2003). PPengaruh Jenis dan Volume Pelarut terhadap Hasil Ekstraksi BHA dan BHT dari minyak goreng.
Noer, S. F. (2011). PENGARUH KADAR ETANOL DALAM SEDIAAN GEL ANTISEPTIKA TERHADAP PERTUMBUHAN BAKTERI Salmonella thyposa. 6.
Permadi, A., Sutanto, & Wardatun, S. (2018). PERBANDINGAN METODE EKSTRAKSI BERTINGKAT DAN TIDAK BERTINGKAT TERHADAP FLAVONOID TOTAL HERBA CIPLUKAN (Physalis angulata L.) SECARA KOLORIMETRI. 1–10.
Ramamurthy, S. (2014). Phytochemical, Pharmacological and Toxicological Properties of Ficus deltoidea: A Review of a Recent Research. Annual Research & Review in Biology, 4(14), 2357–2371. https://doi.org/10.9734/arrb/2014/8281
Samah, O. A., Zaidi, N. T. A., & Sule, A. B. (2012). Antimicrobial activity of Ficus deltoidea Jack (Mas Cotek). Pakistan Journal of Pharmaceutical Sciences, 25(3), 675–678.
Sapara, T. U., & Waworuntu, O. (2016). Efektivitas Antibakteri Ekstrak Daun Pacar Air (Impatiens Balsamina L.) Terhadap Pertumbuhan Porphyromonas Gingivalis. Pharmacon, 5(4), 10–17. https://doi.org/10.35799/pha.5.2016.13968
Silalahi, M. (2019). PEMANFAATAN DAN BIOAKTIVITAS TABAT BARITO (Ficus deltoidea JACK). Jurnal Ilmiah Ilmu Kesehatan: Wawasan Kesehatan, 6(1), 29. https://doi.org/10.33485/jiik-wk.v6i1.155
Suryati, Nurdin, H., & H, Dachriyanus, dan M. N. (2009). PROFIL FITOKIMIA DAN AKTIFITAS ANTIACETYLCHOLINESTERASE DARI DAUN TABAT BARITO (Ficus deltoidea). 2(2), 169–173.
